摘要
Objective:Toinvestigatethepreparationofthecarboxymethlydextranironoxidemagneticnanoparticles(CDMN)andtheeffectsofCDMNcarriersystemassociatedwithexternalmagneticfieldsonkillingtumorcellsandgenetransfectioninvitro.Methods:Epirubicin-CDMN(EPI-CDMN)andgreenfluorescentprotein(GFP)plasmid-CDMN(GFP-CDMN)werepreparedbytheoxidation-reductionprocedureandtheircharactersweredetected,respectively.TheeffectsofEPI-CDMNassociatedwithexternalpulsedelectromagneticfields(PEMFs)(10mT)onkillinghumanbladdercancerBIU-87cellswerestudiedbyMTTassayandAnnexin-V/PIdouble-labeledflowcytometrytechnique,respectively.AndthetransfectionefficiencyofGFPwhenCDMNwereusedasgenecarrierassociatedwiththeexternalmagneticfieldswasevaluatedunderfluorescencemicroscopeinvitro.Results:ThediameterofEPI-CDMNandGFP-CDMNwereabout8~10nmand5~9nm,respectively,andsaturationmagnetizationwere0.22emu/gand0.26emu/g,respectively.EPI-CDMNassociatedwithPEMFscouldsignificantlyinhibittheproliferationofBIU-87cellsandinducecellsapoptosis,thegrowthinhibitoryrateandapoptosisratewere(21.82(3.18)%and(16.79(3.37)%,respectively.ThetransfectionefficiencyofGFP-CDMNcombinedwithPEMFswassignificanthigherthanthatofGFP-CDMNwithoutPEMFs[(45.70(4.32)%vs(35.85(2.16)%,P<0.05].Conclusion:ItseemedthatEPI-CDMNassociatedwithexternalmagneticfieldscouldsignificantlykilledhumanbladdercancerBIU-87cellsandCDMNcouldeffectivelytransferGFPgeneintotumorscellswiththehelpofexternalmagneticfieldswhichprovidedexperimentalbasisforthemagnetictargetingtherapyoftumor.
出版日期
2005年01月11日（中国Betway体育网页登陆平台首次上网日期，不代表论文的发表时间）
