Comparative analysis of volatile oils in the stems and roots of Ephedra sinica via GC-MS-based plant metabolomics-中国Betway体育网页登陆

摘要 WithagreatdifferenceintherapeuticeffectsofMahuang(MH,thestemsofEphedrasinica)andMahuanggen(MHG,therootsofEphedrasinica),chemicaldifferencesbetweenMHandMHGshouldbeinvestigated.Inthepresentstudy,gaschromatography-massspectrometry(GC-MS)-basedplantmetabolomicswasemployedtocomparevolatileoilprofilesofMHandMHG.TheantioxidantactivitiesofvolatileoilsfromMHandMHGwerealsocompared.32differentialchemicalmarkerswereidentifiedaccordingtothevariableimportanceintheprojection(VIP)valueoforthogonalpartialleastsquaresdiscriminantanalysis(OPLS-DA)andPvalueofMann-Whitneytest.Amongthem,chemicalmarkersoftetramethylpyrazine(TMP)andα-terpineolwerequantified.TheircontentsweremuchhigherinmostMHsamplescomparedwithMHG.TheantioxidantassaydemonstratedthatMHhadsignificantlyhigherfreeradical-scavengingactivitythanMHG.AlthoughMHandMHGderivedfromthesamemedicinalplant,therewasmuchdifferenceintheirvolatileoilprofiles.MHsampleshadsignificantlyhighercontentoftworeportedpharmacologicallyimportantchemicalmarkersofTMPandα-terpineol,whichmayaccountfortheirdifferentantioxidantactivities.

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Comparative analysis of volatile oils in the stems and roots of Ephedra sinica via GC-MS-based plant metabolomics

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