简介:目的建立一种简便、快捷、准确的检测方法用于近交系小鼠的遗传检测。方法根据近交系小鼠的H-2基因序列设计相应的探针,并标记生物素,利用微孔板Southern杂交技术,使探针与模板DNA杂交,再加入亲和素标记的辣根过氧化物酶进行酶显色反应,通过酶标仪检测杂交结果,以确定近交系小鼠的基因型。结果57BL/6和C57B:/10为H-2^b型;DBA/2和Scid为H-2^d型;615和C3H为H-2^k型;NCPC/2、TA1、TA2和T739均为H-2^b型。结论通过Southern杂交检测可以确定近交系小鼠的基因型。该检测方法简便、易行,检测结果客观,可以应用于近交系小鼠的遗传检测。
简介:把Niya节的地质的记录基于多索引的合成研究,它具有在Tarim盆的南部的边缘的高分辨率,和在南部的Xinjiang的另外的地质的记录,这篇论文重建了自从大约4.00kaBP,paleoclimatic的历史在这个区域变化。在最后4.00ka期间,南部的Xinjiang的区域经历了相对寒冷湿、相对的温暖干燥的时期的引申。三个显著寒冷湿的时期(4.00-3.45kaBP,2.50-1.90kaBP,ca。1.40?1.00kaBP)并且三个温暖干燥的时期(3.45-2.50kaBP,1.90-1.40kaBP,1.00kaBP礼品)被识别。人的活动在南部的Xinjiang与古气候的进化有一种亲密关系,这被显示出。
简介:SeismicanisotropybeneathSouthernTibetQING-TIANLUI(吕庆田),KAI-YIMA(马开义),MEIJIANG'(姜枚),A.HirnandA.Nercessian(InstituteofMineralDe...
简介:Sedimentaryfaciesisanimportantfactorinfluencingshalegasaccumulation.Itnotonlycontrollshydrocarbongeneration,butalsoaffectsreservoircharacteristicsanddistribution.ThispaperdiscussestheLowerSilurianLongmaxiFormationinthesouthoftheSichuanBasin.Outcrop,core,drillingandloggingdataidentifythesedimentaryfaciesoftheformationascontinentalshelffacies,whichisdividedintotwosubfacies:aninnershelfandanoutershelfsubfacies.Thesetwosubfaciescanbefurtherdividedintosevenmicrofacies:muddysiltyshallowshelf,calcareoussiltyshallowshelf,muddylimyshallowshelf,stormflow,muddydeepshelf,siltymuddydeepshelfandcontourcurrentmicrofacies.Verticalandhorizontaldistributionofmicrofaciesestablishesasedimentationmodelofthecontinentalshelffacies.Combinedwithanalizationorcalculationofgeochemical,mineralogical,physicalandgas-bearingpropertiesofsamples,sedimentarymicrofaciesisevaluatedusingnineparameters:totalorganiccarboncontent,effectiveshalecontinuousthickness,vitrinitereflectance,kerogentype,mineralcomponents,porosity,permeability,watersaturationandgascontent.Theevaluationrevealedthatthemostfavorablefaciesforshalegasexplorationanddevelopmentarethemuddydeepshelfandpartofthesiltymuddydeepshelfmicrofacies,withTOCmorethan2%,siliceouscomponentover50%,claylessthan30%,porositymorethan3%,watersaturationlowerthan40%,gascontentgreaterthan2m3/t.Theseresultsprovideatheoreticalbasisfordeciston-makingonthemostpromisingareasforshalegasexplorationintheSichuanBasinandformarineshalegasexplorationanddevelopmentinSouthChina.
简介:使用每月的风和海面温度(SST)数据,在印度洋与印度洋偶极子模式(IOD)事件联系的南部的南方的大气循环房间第一次被描述并且检验。分叉的风和压力vertical速度为大气循环房间的鉴定被采用。在积极IOD事件的四个不同阶段期间,在西方的印度洋上的异常南方的哈德利发行量证明空气在更低的对流层在热带,在上面的对流层的流动杆病房,在画热带的水池,和回来升起回到热带。在东方印度洋上的塞诺马劳斯·哈德利循环在西方的印度洋上对那相反。在积极IOD事件期间,当它在西方的印度洋上被加强时,在东方IndianOcean上的南方的哈德利发行量被削弱。在IOD索引和哈德利房间的索引之间的相关分析也证明那,大气循环模式在在记录的时期上的每个IOD事件是明显的。
简介:InSeptember2002,"OrganizingofNationalRareEarthEnterprisesGroup"whichwasdeliveredbyoriginalNationalEconomy&TradeCommitteeandoriginalNationalPlanningCommittee,MinistryofFinanceP.R.C,MinistryofLand&ResourceP.R.C.andMinistryofForeignEconomy&Trade,wasapprovedbytheStateCouncilofP.R.C.toorganizeSouthernandNorthernRareEarthGroups.OnOctober30,2002,ChinaNorthernREGroupCo.,LtdPreparationTeamheldthefoundationconventioninBaotou,whichdrewthecurtainof...
简介:TheanalysisoftherelationshipbetweenseaiceinArcticandAntarctic,andtheSouthernOscillationIndex(SOI)variationonthetemporalscaleofmonth,seasonandyearismadeinthispaper.ThenegativecorrelationbetweenseaiceinArcticandtheSOI.andthepositivecorrelationbetweenseaiceinAntarcticandtheSOIarefound.ThevariationofseaiceinAntarcticPacificseemstoplayanimportantroleintheinfluencetoatmosphere.Theirnonlinearrelationshipspresentmorestronglythanthelinearrelationshipbetweenthem.TherelationshipbetweenseaiceinArcticandAntarcticandtheSOIintheearlystageisinvestigated.Theirvariationshowsperiodicphenomena.ThemaximumcorrelationisusedtobuildthemodelforforecastingthevariationoftheSOIontheconditionoftheseaiceinArcticandAntarctic.Theexplainedvarianceofthesimulatedseriesisgreaterthan0.90.Thereexistsa17-monthperiodintherelationshipbetweenseaiceinArcticandAntarcticandSOI.Theexistingofthecommonfactorwithlargerscaleisdiscussedinthispaper.
简介:AbstractObjective:To summarize the main updated evidence about the health effects of air pollution, with a special focus onSouthernEurope.Data sources:Literature was obtained through PubMed Central and the official websites of European Agencies and Scientific Societies.Study selection:Recent shreds of evidence about the health effects of air pollution coming from international reports and original research were collected and described in this review.Results:Air pollution is an avoidable risk factor that causes a huge burden for society, in terms of death, health disorders, and huge socio-economic costs. ThesouthernEuropean countries face a more threatening problem because they experience the effects of both anthropogenic pollutants and natural dusts (particulate matter [PM]). The European Environment Agency reported the number of premature deaths in the 28 countries of the European Union attributable to air pollutant exposure in the year 2016: 374,000 for PM2.5, 68,000 for nitrogen dioxide, and 14,000 for ozone. In Italy, time series and analytical epidemiological studies showed increased cardiorespiratory hospital admissions and mortality, as well as increased risk of respiratory diseases in people living in urban areas.Conclusions:Based on abundant evidence, the World Health Organization, which hosts the Global Alliance against Chronic Respiratory Diseases (GARD), the scientific respiratory societies, and the patients’ associations, as well as others in the health sector, must increase their engagement in advocacy for clean air policies.