摘要
InordertoidentifythepotentialnephrotoxiccompoundsintraditionalChinesemedicineLithospermumerythrorhizon,itwasseparatedintoserialfractionsaccordingtotheirpolarities.Aninvitromethodwasutilizedtodeterminethenephrotoxicityofthesefractionswiththehelpoffluorescenceimageanalysis.Asaresult,theprimaryfractionA05anditssecondaryfractionsC06'C09andC12'C14werefoundtohavesignificanttoxicitytoLLC-PK1cellline,asdeterminedbythesurviveratelessthan20%aftertheyweretreatedwiththesefractions.Thesepotentialnephrotoxicfractionswerefurtheranalyzedbymultistageandhighresolutionmassspectrometry.Themaincompoundsinthesefractionsweretentativelyidentifiedtobeacetylshikonin,isobutyrylshikonin,β,β'-dimethyla-cryloylshikonin,andisovalerylshikonin,whichmaybringnephrotoxicity.
出版日期
2011年04月14日（中国Betway体育网页登陆平台首次上网日期，不代表论文的发表时间）
