Cloning and Expression Analysis of a Mitogen-Activated Protein Kinase Gene OsMPK14 in Rice-中国Betway体育网页登陆

摘要 Mitogenactivated-proteinkinases(MAPKs)areimportantcomponentsinsignaltransductionpathwaysrespondingtovariousbioticandabioticstresses.AnMAPKgene,OsMPK14(GenBankAccessionNo.GQ265780)fromrice(OryzasativaL.),wasclonedbyRT-PCR.Thefull-lengthcDNAofOsMPK14consistsof1660bpinsize,containinganopenreadingframeof1629bp,whichencodesa542-amino-acidpolypeptideandhasatypicalproteinkinasedomainandaphosphorylationactivationmotifTDY.SequencealignmentandanalysisrevealedthatOsMPK14waslocatedonricechromosome5,andcomposedofnineexonsandeightintronsinthecodingregion.Semi-quantitativeRT-PCRwasperformedtodetecttheexpressionpatternsofOsMPK14inriceshootsandrootsunderdarkness,drought,highsalinity,lowtemperatureandabscisicacidtreatments.TheOsMPK14mRNAwasinducedbyabscisicacid,lowtemperatureandhighsalinity,butweaklyinhibitedbydrought.Inaddition,theexpressionofOsMPK14wasup-regulatedinroots,butdown-regulatedinshootsbylight.TheresultsindicatethatOsMPK14couldbeimplicatedindiversericestimuli-responsivesignalingcascades,anditsexpressionmightberegulatedbymultiplefactors.

1Xu Jia-Li,Wang Xin-Zhu,Jiang Hu-Ning,Chen Yi,Wang Rong,Shu Yong-Qian.Combination therapies with mitogen-activated protein kinase kinase inhibitors and immune checkpoint inhibitors in non-small cell lung cancer.医药卫生,2020-11.
2CHEN Jing;WAN Jia;JIANG Hua;GAO Xiao-ling;WANG Ping-rong;XI Jiang;Xu Zheng-jun.Cloning and Expression Analysis of OsNADPH1 Gene from Rice in Drought Stress Response.作物学,2006-03.
3GuangxiaoYang SetsukoKomatsu.Microarray and Proteomic Analysis of Brassinosteroid-and Gibberellin-Regulated Gene and Protein Expression in Rice.分子生物学,2004-02.
4于金贵.The inhibitory effects of sevoflurane on angiotensin Ⅱ-induced, p44/42 mitogen-activated protein kinase-mediated contraction of rat aortic smooth muscle.麻醉学,2005-02.
5.Corrigendum: Follistatin-Like 1 Promotes Bleomycin-Induced Pulmonary Fibrosis Through the Transforming Growth Factor Beta 1/Mitogen-Activated Protein Kinase Signaling Pathway.医药卫生,2020-08.
6Ruixi Zhou,Junjie Ying,Xia Qiu,Luting Yu,Yan Yue,Qian Liu,Jing Shi,Xihong Li,Yi Qu,Dezhi Mu.A new cell death program regulated by toll-like receptor 9 through p38 mitogen-activated protein kinase signaling pathway in a neonatal rat model with sepsis associated encephalopathy.医药卫生,2022-12.
7Hong-Kun Yu;Hao Ghen;Yong-Jun Zhang;Kong-Ming Wu;Ge-Mei Liang;Ze-Wen Liu;Yu-Yuan Guo.Gene cloning and expression of aminopeptidase N and cadherin from midgut of the rice stem borer, Chilo suppressalis.昆虫学,2010-05.
8Danhua Jiang;Changsong Yin;Aiping Yu;Xiaofan Zhou;Wanqi Liang;Zheng Yuan;Yun Xu;Qingbo Yu;Tieqiao Wen;Dabing Zhang.Duplication and expression analysis of multicopy miRNA gene family members in Arabidopsis and rice.细胞生物学,2006-05.
9Na Zhu,Min Han.Roles of adenosine monophosphate-activated protein kinase (AMPK) in the kidney.医药卫生,2022-12.
10Yu GANG WANG;KUN PENG ZHAO;Ju GAO CHEN;YAN LI;YUANG FANG MA;BEI FEN SHEN.Cloning, expression and function of the extracellular fragment of human TRAIL gene.基础医学,2007-02.

Cloning and Expression Analysis of a Mitogen-Activated Protein Kinase Gene OsMPK14 in Rice

来源期刊

相关推荐

同分类资源更多

相关关键词