摘要
Inthepresentstudy,27multi-drugresistantstrainsofPseudomonasaeruginosawereisolatedfromclinicalspecimensinourhospitalfromJan2005toNov2005,inwhichtheresistantgenesencodingβ-lactamaseincludingTEM,SHV,OXA,PER,VEB,GES,CARB,IMP,VIM,SPM,GIM,DHAandOprD2weretestedbyPCRamplificationandsequencedbyDNAsequencer.Itwasfoundthatthedetectionratesofbla_(VEB),bla_(GES)andbla_(CARB)genesinthese27isolatesofP.aeruginosawere11.1%,11.1%and48.1%,respectively,butalmosttheoprD2genewaslacked(92.6%).Inaddition,theresistantgenesencodingTEM,SHV,OXA,PER,IMP,VIM,SPM,GIMandDHAβ-lactamasewereallnotfound.Itwasalsodemonstratedthatthesequenceofbla_(VEB)geneappearedtobeidenticaltothatofthebla_(VEB-1)(AY536743),whilethebla_(CES)andbla_(CARB)genesshared99%identitywithbla_(GES-1)(AY219651)andbla_(CARB-3)(S46063)genes.Fromtheseobservations,itisevidentthatP.aeruginosacarryingthebla_(VES),bla_(GES)andbla_(CARB)resistantgenesisolatedinourhospitalconferstheresistancetoβ-lactams,andthelossoftheoprD2genemaybetheimportantcausetodevelopresistancetoimipeneminP.aeruginosa.
出版日期
2007年01月11日（中国Betway体育网页登陆平台首次上网日期，不代表论文的发表时间）
