Ectopic bone formation of human bone morphogenetic protein-2 gene transfected goat bone marrow-derived mesenchymal stem cells in nude mice-中国Betway体育网页登陆

摘要 Objective:Toevaluatetheosteogenicpotentialofbonemorphogeneticprotein(BMP)-2genetransfectedgoatbonemarrow-derivedmesenchymalstemcells(MSCs).Methods:Goatbonemarrow-derivedMSCsweretransfectedbyAdv-humanbonemorphogeneticprotein(hBMP)-2gene(Group1),Adv-betagaltransfectedMSCs(Group2)anduninfectedMSCs(Group3).Westernblotanalysis,alkalinephosphatasestaining,VonKossastainingandtransmissionelectronmicroscopywereadoptedtodeterminethephenotypeofMSCs.Thenthecellswereinjectedintothighmusclesofthenudemice.Radiographicalandhistologicalevaluationswereperformedatdifferentintervals.Results:OnlyAdv-hBMP-2transfectedMSCsproducedhBMP-2.Thesecellswerepositiveforalkalinephosphatasestainingatthe12thdayandwerepositiveforVonKossastainingatthe16thdayaftergenetransfer.Electronmicroscopicobservationshowedthatthereweremoreroughendoplasmicreticulum,mitochondriaandlysosomesinAdv-hBMP-2transfectedMSCscomparedtoMSCsofothertwogroups.Atthe3rdand6thweeksaftercellinjection,ectopicboneswereobservedinmusclesofnudemiceofGroup1.Onlyfibroustissueoralittlebonewasfoundinothertwogroups.Conclusions:BMP-2genetransfectedMSCscandifferentiateintoosteoblastsinvitroandinduceboneformationinvivo.

1Xiao-Huang Tu Jing-Xiang Song Xiao-Jun Xue Xian-Wei Guo Yun-Xia Ma Zhi-Yao Chen Zhong-Dong Zou Lie Wang.Role of bone marrow-derived mesenchymal stem cells in a rat model of severe acute pancreatitis.消化系统,2012-12.
2Guangwu Xu Liying Zhang Guangwen Ren Zengrong Yuan Yingyu Zhang Robert C Zhao Yufang Shi.Immunosuppressive properties of cloned bone marrow mesenchymal stem cells.细胞生物学,2007-03.
3杨立业;郑佳坤;汪朝阳;李文玉.Differentiation of adult human bone marrow mesenchymal stem cells into Schwann-like cells in vitro.临床医学,2005-02.
4XU Fang.A novel, truncated human bone morphogenetic protein-2:construction, expression ,functions and clinical potential.生物医学工程,2001-03.
5Neng-Sheng Ye Rong-Li Zhang Yan-Feng Zhao Xue Feng Yi-Ming Wang Guo-An Luo.Effect of 5-azacytidine on the Protein Expression of Porcine Bone Marrow Mesenchymal Stem Cells in vitro.分子生物学,2006-01.
6TANG,Lanyan.Therapeutic Effect of Bone Marrow Mesenchymal Stem Cells on Rat Bladder Cancer.,2023-03.
7盛小刚;宋卉;冯建章;陈秋雄.Differentiation of Rabbit Bone Marrow Mesenchymal Stem Cells to Myogenic Ceils and Expression of VEGF After Gene Transfection.心血管疾病,2005-02.
8LI Xiaoyan;WANG Hao;YANG Yang;TAN Min;XUE Jingya;NI Haidong;GUO Yajun.Recombinant human bone morphogenetic protein-7 expressed from CHO cells possessing the activity of bone-induced in vitro.骨科学,2006-03.
9秦凤华;蒋激扬;李爱玲;金永柱;郝洁;谢蜀生.Acceleration of Immune Reconstitution after Bone Marrow Transplantation in Mice by Bone Marrow Stromal.基础医学,2003-01.
10Yan Liu Yingdong Zhang.Distribution and differentiation of bone marrow-derived mesenchymal stem cells in vivo after intraperitoneal and tail vein injection into rats in the recovery phase of stroke Which path is better？.神经病学与精神病学,2010-12.

Ectopic bone formation of human bone morphogenetic protein-2 gene transfected goat bone marrow-derived mesenchymal stem cells in nude mice

来源期刊

相关推荐

同分类资源更多

相关关键词