摘要
OnthebasisoftherandomamplifiedpolymorphicDNA-fingerprinting(RAPD)method,10morphospeciesofscuticociliatesfrom7genera,including15clonesof13strains,Uronemarnarinum,Uronemacfmarinum,Parauronernavirginianum,P.longurn,Metanophryssimilis,M.sinensis,Paralembusdigitiformis,Mesanophryscarcini,ParanophrysmagnaandCohnilembusverminuswereanalysedusing8oligonucleotideprimers.Thegeneticsimilarityamongtheclonesofthesamestrainmeasuredbyaband-sharingindexis0.97～0.98,while0.40～0.52amongstrains.Thisvaluemeasuredis0.39～0.46amongcongenersofthesamegenus,whereas0.16～0.47betweendifferentgenera.Adistancetreewasconstructedbasedon8-primeranalysis,inwhichthescuticociliatesinvestigatedwereseparatedintotwoclusters:oneconsistsof2genera,UronemaandParauronema,andtheotherwascomposedoffive,Metanophrys,Mesanophrys,Paranophrys,ParalembusandCohnilembus.ItisdemonstratedalsothatthemorphospeciesParauronemavirginianummaybeaspecies-complex,i.e.,itcontainsdifferentgenospecies.
出版日期
2005年03月13日（中国Betway体育网页登陆平台首次上网日期，不代表论文的发表时间）
