Establishment of a Two-site ELISA for Detection of Chlamydia Trachomatis

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    摘要 Objective:ToestablisharapidandsimpleassayforthediagnosisofChlamydiatrachomatis(CT)infection.MethodsBALB/cmicewereimmunizedwithSDS-PAGEpurifiedmajoroutermembraneprotein(MOMP)fromCTandthemonoclonalantibodieswereobtainedsubsequently.Two-siteELISAwasdevelopedtodetectCTinfection.Results:Theestablishedassaywasabletodetectaslowas1.248ug/mlMOMPwithinterrunandinrunCV6.9%and3.1%respectively.94%(34/36)ofculture-positivesampleswerefoundtobepositiveinthecurrentexamination,indicatingthehighsensitivityofthisassay.Conclusion:TheassayisapplicableforclinicaldiagnosisofCTinfection.
    机构地区 不详
    出版日期 2002年01月11日(中国Betway体育网页登陆平台首次上网日期,不代表论文的发表时间)
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