Multiplication of Elaeagnus angustifolia L. from a Single Shoot in Vitro

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    摘要 MultiplicationofElaeagnusangustifoliaL.wasexaminedinvitrosuccessivelyfromasingleshootunderthespecifiedconditionofdifferentmedia,plantgrowthregulators,pHvalueandsucroseconcentration.ItwasshowedthatMMS1wasthemostsuitablemediumonshootmultiplicationamong5mediaconcerned;BAPwasthemosteffectiveoneamongallthecytokinininvolved,BAP,KN,TDZandZT;theexpluntofthetophalf-partfromashootproducedmorenewshootsthanthatofthefoothalf-partdid;morenewshoots(>2cm)wereproducedunder3%sucrosebetweentheconcentrationswithtophalf-partexplants;shootscouldgrowwellbetweenpH4.4andpH7.0,andthebiggestnumberofshootswasproducedinpH5.6,whileinpH5.8themaximumrootingrateappeared.Asaresult,thecombinationof0.5μMBAPand0.1μMIBAonMMS1mediuminducedthemaximumshootmultiplication.Thenumberofshootamplified3timesin1month,and3^12shoots(>2cm)mightbetheoreticallymultipliedannuallyfromasingleshoot.
    机构地区 不详
    出版日期 2004年01月11日(中国Betway体育网页登陆平台首次上网日期,不代表论文的发表时间)
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