摘要
Aim:ToevaluatetheantifertilityeffectofAlstoniascholarisbarkextractinmalerats.Methods:InmaleWistarratsAlstoniascholarisbarkextractwasgivenbyoralrouteatadoseof200mg/dayfor60days,Thefertilityandtesticularfunctionwereassessedbymatingtests,spermmotility,spermconcentration,biochemicalindicesandtesticularcellpopulationdynamics.Results:Oralfeedingwiththeextractatadoseof200mg/dayfortheperiodof60daysdidnotcausebodyweightloss,whiletheweightsoftestes,epididymides,seminalvesicleandventralprostateweresignificantlyreduced.Theproductionofstep-19spermatidswasreducedby79.6%intreatedrats.Thepopu-lationofpreleptoteneandpachytenespermatocytesweredecrasedby61.9%and60.1%,respectively.Spematogo-niaandSertolicellpopulationwerealsoaffected.TheseminiferoustubuleandLeydigcellnuclearareawerereducedsignificantly(P<0.01)whencomparedtothecontrols..Reducedspermcountandmotilityyesultedinatotalsupprs-sionoffertility.Asignificantfallintheproteinandsialicacidcontentofthetestes,epididymides,seminalvesicleandventralprostateaswellasglycogencontentoftesteswerealsonoticed.Thefructosecontentintheseminalvesiclewasloweredwhereasthetesticularcholesterolwaselevatedascomparedwiththecontrols.Thefollowingcom-poundswereobtainedfromtheextractwithchromatographicseparationoverSi-gelcolumn;α-amyrin,β-amyrin,lupiolacetate,venenative,rhazineandyohimbine,Conclusion:Atthedoselevelemployed,Alstoniascholarisbarkextracthasasignificantantifertilityeffectinmalerast;theprimarysiteofactionmaybepostmeioticgermcells(Step19spermatids).
出版日期
2002年03月13日（中国Betway体育网页登陆平台首次上网日期，不代表论文的发表时间）
