Simplified heavy metal staining techniques demonstrated with Fast Plant leaf tissue-中国Betway体育网页登陆

摘要 FastPlant(Brassicarapa,Cruciferae)leaftissuefixedinglutaradehyde-acroleinandpost-fixedinosmium,wasexaminedforresponsetoseveraleasilypreparedheavymetalstains.Leadanduranium,separatelyandincombination,gavetypicalresultsacrossthespectrumofcellorgeanelles.Asssinglestainfollowingosmium,bismuthproducedimagesseeminglyequivalenttoleadanduranium.Phosphotungsticacidproducedverygoodmembranedelineationbutproducedawashed-outbackgroundimagesimilartothatfromleadstaining.Carbohydratecompoundswereespeciallyresponsivetoruthenium;thecytoplasmandthematrixofallorganelleswerealsostainedverywell.Theprocedureswerenomoredemandingthantraditionalstainingmethodsandmaybeeasilyusedinresearchandteaching.FastPlantmaterialsareareliable,quicknandeasysourceoflivingmaterial.

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Simplified heavy metal staining techniques demonstrated with Fast Plant leaf tissue

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