Chemical synthesis of a structural gene coding for trichosanthin-中国Betway体育网页登陆

摘要 Astructuralgene(750bp),whichcodesforatypeIribosomeinactivatingprotein,trichosanthin,hasbeendesignedaccordingtothecodonusageofhighlyexpressedgeneinE.coliandchemicallysynthesized.Inthesynthesizedgene,twenty-sevenuniquerestrictionsiteswereevenlydispersedwithanaveragedistancebetweentwoadjacentsiteslessthan50bptofacilitateasystematicinvestigationonstructure-functionalrelationshipofthisproteinbysite-directedmuta-genesis.Tosynthesizeit,thewholegenewasdividedintothreelargefragments(EP,PNandNH)whichwereassembledfromseveralchemicalsyntheticoligonucleotidesbyenzymaticmethod.TheassemblyofboththefragmentEPfromsixoligonucleotides(A-F)andthefragmentPNfromfouroligomers(G-J)wascatalyzedbyT4DNAligaseinusingthesinglestrandedDNAmethod[Chen,H.-B.etal,Nucl.AcidsRes.,18,871(1990)].AndfragmentNHwasformedfromthreeduplexesK,LandMbytheclassicaldoublestrandedDNAmethod.Finally,

1HUANG Lin-Fang;WANG Zeng-Hui;CHEN Shi-Lin.Hypericin： chemical synthesis and biosynthesis.中药学,2014-02.
2G. Praveena;A. Abiram.Structural and Interaction Properties of Porphyrin Layers A Quantum Chemical Study.物理,2015-05.
3Yu, RM;Lu, SF;Huang, XY;Huang, JQ.Synthesis and structural characterization of a new trimetallic thiocubane cluster.化学,1997-02.
4Nadir F. Habubi;Noor A. Nema;Abdulazeez O. Mousa.Synthesis and Characterization of ZnO Nanostructure by Chemical Spray Pyrolysis Method.物理,2016-01.
5莫家庆;冯志强;侯军伟;贾振红;吕小毅.Synthesis and photoluminescence properties of CdS hollow nanospheres by chemical vapor deposition.物理电子学,2011-03.
6Xiong Lingyuan Lin Tao Zhou Hantao Xu Jinsen Ge Yunsheng Chen Muchuan Chen Liang.Cloning and Structural Analysis of Calmodulin Gene from the Mangrove Plant Sonneratia Paracaseolaris.海洋科学,2002-02.
7.One—Step Synthesis of MIBK from Acetone Developed by Jilin Chemical Company.油气加工工程,2002-03.
8YANGHuaming ZHANGXiangchao YANGWuguo HUANGChenghuan QIUGuanzhou.Chemical synthesis of nanocrystalline ZrO2-Sn02 composite powders.金属材料,2004-02.
9Sheetal;V.B.Taxak;Rajni Arora;Dayawati;S.P.Khatkar.Synthesis, structural and optical properties of SrZrO3：Eu3＋phosphor.有色金属冶金,2014-04.
10Seyed Hesam Mousavi;Shohreh Fatemi;Marian Razavian.Synthesis and stability evaluation of hierarchical silicoaluminophosphates with different structural frameworks in the methanol to olefins process.力学,2018-02.

Chemical synthesis of a structural gene coding for trichosanthin

来源期刊

相关推荐

同分类资源更多

相关关键词