Combined transfection of Bcl-2 siRNA and miR-15a oligonucleotides enhanced methotrexate-induced apoptosis in Raji cells-中国Betway体育网页登陆

摘要 Objective:B-celllymphoma2(Bcl-2)isanimportantmemberoftheBcl-2familyofproteinsthatregulatetheinductionofapoptosis.ThisstudyaimstoinvestigatewhetherBcl-2smallinterferingRNA(siRNA)combinedwithmiR-15aoligonucleotides(ODN)couldenhancemethotrexate(MTX)-inducedapoptosisinRajicells.Methods:ChemicallysynthesizedmiR-15aODNandBcl-2siRNAweretransfectedinRajicellsbyusingaHiPerFectTransfectionReagentandthencombinedwithMTX.ExpressionlevelsofBcl-2proteinweredetectedbyWesternblot.CellproliferationwasdeterminedbyCCK8assay.TherateofcellapoptosiswasdeterminedbyAnnexinV/PIdoublestaining.ThemorphologyofapoptoticcellswasobservedbyHoechst-33258staining.Results:AfterthecellsweretransfectedwithmiR-15aODNcombinedwithBcl-2siRNA,Bcl-2proteinlevelswereevidentlydecreased.CCK8assayshowedthatcellproliferationwassignificantlydecreasedandwassignificantlylowerinmiR-15aODNcombinedwithBcl-2siRNAplusMTXgroupthaninmiR-15aODNwithmethotrexategroup,Bcl-2siRNAwithMTXgroup,andsingleMTXgroup(P<0.05).Hoechst33258stainingrevealednumerousapoptoticcells.AnnexinV/PIdoublestainingshowedthattheapoptoticrateswere(13.13±1.60)%,(34.47±2.96)%,(32.87±3.48)%,and(45.47±2.16)%inMTX,Bcl-2siRNAplusMTX,miR-15aODNplusMTX,andmiR-15aODNcombinedwithBcl-2siRNAplusMTXgroups,respectively.Amongthesegroups,theapoptoticrateofmiR-15aODNcombinedwithBcl-2siRNAplusMTXgroupwasthehighest;thisapoptoticratewasalsosignificantlydifferentfromthatofmiR-15aODNorBcl-2siRNAplusMTX(P<0.05).Conclusions:Bcl-2siRNAcombinedwithmiR-15aODNcouldenhanceMTX-inducedapoptosisinRajicells.Bcl-2siRNAandmiR-15acombinedwithMTXmaybeausefulapproachtoimprovethetreatmenteffectsonlymphoma.更多还原

1ZHANG Shou-De;SHAN Lei;LI Wei;LI Hong-Lin;ZHANG Wei-Dong.Isochamaejasmin induces apoptosis in leukemia cells through inhibiting Bcl-2 family proteins.中药学,2015-09.
2GuoBC;XuYU.Bcl－2 over－expression and activation of protein kinase C suppresss the Trail－induced apoptosis in Jurkat T cells.细胞生物学,2001-02.
3JIA HE WANG;YI JUN ZHOU;NA MA;LI MIN ZHENG;BAI YI CHEN.Differential expression of bcl-2 and bax genes during the E. coli-induced apoptosis of human U937 cells.基础医学,2005-04.
4郑军;姚榛祥;张静.THE EXPRESSION AND CLINICAL VALUE OF APOPTOSIS CONTROL GENE Bcl-2 AND Bax IN BREAST CANCER.肿瘤,1999-03.
5ZHANGMIN;HONGQINGZHANG;等.Effect of Bcl—2 and caspase—3 on calcium distribution in apoptosis of HL—60 cells.细胞生物学,2000-03.
6何冬梅;张洹.ENHANCEMENT OF RADIATION-INDUCED APOPTOSIS IN RAJI CELL LINE BY BC1-2 ANTISENSE OLIGODEOXYNUCLEOTIDE.肿瘤,2005-02.
7胡海燕;张洹.siRNA对肺癌细胞株bcl-2基因表达的抑制（摘要）.肿瘤,2005-02.
8崔玉芳;夏国伟;付小兵;杨红;彭瑞云;张莹;谷庆阳;高亚兵;崔雪梅;胡文华.Relationship between expression of Bax and Bcl-2 proteins and apoptosis in radiation compound wound healing of rats.临床医学,2003-03.
9Sheng Zhi Wang.Apoptosis induced by hyperthermia in BCACD885 cells.口腔医学,2008-03.
10XU YInglong;LIU Hui;WANG Junli;XU Min.EFFECT OF LIPOSOMAL TRANSFECTION OF HTERT ON APOPTOSIS OF NEWBORN RAT COCHLEAR BASILAR MEMBRANE CELLS.耳鼻咽喉科,2012-02.

Combined transfection of Bcl-2 siRNA and miR-15a oligonucleotides enhanced methotrexate-induced apoptosis in Raji cells

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