摘要
ObjectToexploretheproceduresinper-operativeharvestingandmanagementoffreshhumancochlearspecimensforresearch.MethodsDuringtrans-cochlearsurgerytoremovelargepetro-clivalmeningiomascausinglife-threateningcompressiononthebrainstem,cochleaearenormallydestroyedanddrilledawayinordertoreachtheapicalpetrousandclivusregion.Insteadthecochleacanbedissectedoutafterethicalper-missionwasobtainedfromthelocalethicalcommittee(EPN)andallowancegainedfromthepatients.Sur-geryisperformedbyateamconsistingofoto-andneurosurgeonsasatwo-dayprocedurewithtotalpetro-sectomyincombinationwithaninferiorre-routingofthefacialnerve.Fixationofthecochleaewasdoneintheoperatingroomassoonasthespecimenshadbeenseparatedfromthetemporalbones.Decalcificationbeganafterhours’toovernight’sfixationfor4weeks.Sectioningparalleltothemodiolus(mid-modiolus)wasperformedwithacryostatmicrotome.Thesectionsweresubjectedtoimmunofluorescence(IF).ResultsUsingfreshlyprepared4%paraformaldehyde(PFD)solution,adequatefixationoffineinnerearstructureswasachievedwithhours’immersionofthecochlearspecimens.Decalcificationin6.2%ethylenedi-amine-tetraceticacid(EDTA)solutionfor4weeksyieldedathoroughlydecalcifiedcochlea.Experiencesinprocessing14humancochleaeandanalysingmainlandmarksinfivehumaninnerearplastic/siliconecastsshowedthattheovalwindow/stapesfootplatearebackwardtilted,atanangleabout15degrees,fromtheplaneperpendiculartothemodiolaraxis.Thedistancefromthemodiolarapextotheanteriorborderoftheovalwindow/footplateintheseinnerearcastsmeasuredbetween4and5mm.HighqualityIFstainingwasobtained.ConclusionSurgicallyobtainedhumancochlearspecimen,whenproperlyprocessed,containside-allypreservedantigenicityforimmunohistochemicalstudy.Adequateorientationduringsectioninghelpsob-tainoptimalmid-modiolarsectionsshowingfi
出版日期
2014年01月11日（中国Betway体育网页登陆平台首次上网日期，不代表论文的发表时间）
